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KMID : 0363320130340020204
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Journal of Korean Oriental Internal Medicine
2013 Volume.34 No. 2 p.204 ~ p.214
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Anti-inflammatory activity of the water extract of Polygala tenuifolia Willd
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Oh Hyun-Suk
Kim Byoung-Woo
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Abstract
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Objectives : This study was designed to investigate the cellular and molecular mechanisms of anti-inflammatory activity
of the water extract of Polygala tenuifolia Willd. (Pt-WE).
Methods : Using lipopolysaccharide (LPS)-stimulated murine RAW264.7 cells, we examined inflammatory mediators such
as nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2). Also, the inhibitory effect of Pt-WE on the activity of activator protein 1 (AP-1) and upstream signaling molecules was evaluated. To assess the protective effect of Pt-WE on hydrochloride/ethanol (HCl/EtOH)-induced gastric ulcer in mice, we compared Pt-WE (200 mg/kg) with ranitidine (50 mg/kg) treated mice's gastric mucosa, based on gross observations.
Results : Pt-WE inhibited LPS-induced production of NO, PGE2 in a dose-dependent manner, without causing cytotoxicity.
Pt-WE suppressed AP-1 activation by reducing generations of both c-Jun and c-Fos. In addition, Pt-WE inhibited the p-MKK 4/7 (mitogen-activated protein kinase kinase 4/7) and p-JNK (c-Jun N-terminal kinase) 1 in LPS-stimulated RAW264.7 cells. HCl/EtOH-induced gastric ulcer lesions were inhibited by pre-treatment of Pt-WE based on gross observations. In addition, Pt-WE decreased the phosphorylation level of JNK.
Conclusions : These results demonstrate that Pt-WE has anti-inflammatory and gastroprotective effects.
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KEYWORD
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Polygala tenuifolia, anti-inflammation, activator protein 1 (AP-1), signaling pathway, animal experiment
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